Yintoni omele uyithathele ingqalelo xa usebenzisa i-BOD analyzer:
1. Ukulungiselela phambi kovavanyo
1. Vula unikezelo lwamandla lwe-incubator ye-biochemical kwiiyure ezi-8 phambi kovavanyo, kwaye ulawule ubushushu ukuba busebenze ngokuqhelekileyo kwi-20 ° C.
2. Faka amanzi ovavanyo lokuxutywa, amanzi okugonywa kunye ne-inoculation dilution yamanzi kwi-incubator kwaye uwagcine kwiqondo lokushisa elingaguqukiyo ukuze lisetyenziswe kamva.
2. Isampulu yonyango lwangaphambili
1. Xa ixabiso le-pH lesampula yamanzi alikho phakathi kwe-6.5 kunye ne-7.5; kuqala ukuqhuba uvavanyo olwahlukileyo ukugqiba umthamo ofunekayo we-hydrochloric acid (5.10) okanye isisombululo se-sodium hydroxide (5.9), kwaye emva koko ungathathi isampuli, kungakhathaliseki ukuba kukho imvula. Xa i-acidity okanye i-alkalinity yesampuli yamanzi iphezulu kakhulu, i-alkali ephezulu yokugxilwa kwe-alkali okanye i-asidi ingasetyenziselwa ukungathathi hlangothi, ukuqinisekisa ukuba inani alikho ngaphantsi kwe-0.5% yomthamo wesampulu yamanzi.
2. Kwiisampuli zamanzi eziqulethe ixabiso elincinci le-chlorine yamahhala, i-chlorine yamahhala iya kunyamalala ngokubanzi emva kokushiya iiyure ezingama-1-2. Kwiisampuli zamanzi apho i-chlorine yamahhala ayinakunyamalala kwixesha elifutshane, umlinganiselo ofanelekileyo wesisombululo se-sodium sulfite unokongezwa ukususa i-chlorine yamahhala.
3. Iisampulu zamanzi eziqokelelwe kumanzi anamaqondo asezantsi obushushu bamanzi okanye amachibi e-eutrophic kufuneka afudunyezwe ngokukhawuleza ukuya kuma-20°C ukukhupha ioksijini enyibilikisiweyo ene-supersaturated kwiisampuli zamanzi. Ngaphandle koko, iziphumo zokuhlalutya ziya kuba ziphantsi.
Xa uthatha iisampulu kwiindawo zamanzi ezinamaqondo aphezulu okushisa kwamanzi okanye iindawo zokukhupha amanzi amdaka, kufuneka zipholile ngokukhawuleza malunga ne-20 ° C, ngaphandle koko iziphumo zokuhlalutya ziya kuba phezulu.
4. Ukuba isampuli yamanzi ukuba ivavanywe ayinayo i-microorganisms okanye i-microbial eyaneleyo, isampuli kufuneka ifakwe i-inoculated. Njengolu hlobo lulandelayo lwamanzi amdaka akwimizi-mveliso:
a. Amanzi amdaka akwimizi-mveliso angakhange acocwe ngokwebhayoloji;
b. Ukushisa okuphezulu kunye noxinzelelo oluphezulu okanye amanzi amdaka ahlambulukileyo, ingqwalasela ekhethekileyo kufuneka ihlawulwe kumanzi amdaka avela kumashishini okulungisa ukutya kunye nogutyulo lwasekhaya olusuka kwizibhedlele;
c. Amanzi amdaka amashishini aneasidi kakhulu kunye nealkaline;
d. Amanzi amdaka emizi-mveliso anexabiso eliphezulu le-BOD5;
e. Amanzi amdaka emizi-mveliso aqulethe izinto eziyityhefu ezifana nobhedu, i-zinc, ilothe, i-arsenic, i-cadmium, i-chromium, i-cyanide, njl.
La manzi amdaka angentla kwimizi-mveliso afuna ukucocwa ngee-microorganisms ezaneleyo. Imithombo ye-microorganisms yile ilandelayo:
(1) Ugutyulo lwamanzi amdaka angacocwanga lwasekhaya lubekwe kuma-20°C iiyure ezingama-24 ukuya kwezingama-36;
(2) Ulwelo olufunyenwe ngokucoca isampuli ngephepha lokucoca emva kokuba uvavanyo lwangaphambili lugqityiwe. Olu lwelo lunokugcinwa kwi-20℃ inyanga enye;
(3) Amanzi amdaka aphuma kwiindawo zokucocwa ilindle;
(4) Amanzi omlambo okanye echibi anamanzi amdaka asezidolophini;
(5) Iintlobo zebhaktheriya ezibonelelwe ngesixhobo. Ubunzima be-0.2g ye-bacterial strain, uthele kwi-100ml yamanzi ahlambulukileyo, ugxobhoze ngokuqhubekayo de iziqhumane zihlakazeke, zifake kwi-incubator kwi-20 ° C kwaye zivumele ukuba zime iiyure ezingama-24-48, emva koko uthathe i-supernatant.
Ixesha lokuposa: Jan-24-2024